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人才队伍

韩先干

 

研究领域:微生物致病机理、动物疫苗及动物疫病检测方法研究
电话/传真:021-34293412
电子邮件:hanxgan@163.com  hanxgan@shvri.ac.cn

 

简历

韩先干,男,博士,研究员,1977年出生,安徽肥西人。
中国农业科学院硕士研究生导师;
中国畜牧兽医学会兽医公共卫生分会副秘书长;
2000年安徽农业大学兽医本科毕业;                   
2000年至2002年在安徽省肥西县动物疫病防控中心工作;
2005年华南农业大学预防兽医学硕士毕业;
2008年南京农业大学预防兽医学博士毕业;
2008年8月起在中国农业科学院上海兽医研究所工作;
2013.08-2014.08美国宾夕法尼亚大学国家公派访问学者。
主要从事猪链球菌2 型、禽致病性大肠杆菌、鸭疫里氏杆菌和布鲁氏菌等细菌病的致病机理研究及疫苗研发。主持国家自然科学基金3项、上海市科技兴农重点攻关项目1项、中央级公益性科研院所基本科研业务费专项3项,参加课题10项。获得中国农业科学院科学技术成果二等奖1项。2009年以来,在国内外核心期刊发表论文80余篇,第1及通讯作者论文24篇,其中SCI论文37篇(第1作者12篇),获得国家发明专利6项。

 

获奖及荣誉:
“鸭疫里默氏杆菌毒力基因、诊断技术研究及应用” 获得中国农业科学院科学技术成果二等奖1项(第3完成人)

 

承担科研项目情况:
1.国家自然科学基金青年面上连续项目“禽致病性大肠杆菌自诱导信号分子AI-2的摄取及其调控机制研究” (项目编号:31370045),起止年限:2014.01-2017.12;项目经费:75万
2.国家自然科学基金面上项目“布鲁氏菌LuxS/AI-2型密度感应系统的构建及其调控机制研究” (项目编号:31572546),起止年限:2016.01-2019.12;项目经费:78万
3.上海市科技兴农重点攻关项目“禽致病性大肠杆菌aroA缺失株多价菌蜕疫苗的研制” (项目编号:沪农科攻字(2015)第1-9号)起止年限:2015.05-2018.04;项目经费:90万。
4. 国家自然科学基金青年基金项目“信号分子AI-2对禽致病性大肠杆菌的调控作用研究”(项目编号:31001078),起止年限:2011.01-2013.12;项目经费:19万。(已结题)
5. 中央级公益性科研院所基本科研业务费“牛布鲁氏菌体内感染因子与小鼠巨噬细胞互作研究” (项目编号:2013JB04)起止年限:2013.01-2014.12;项目经费:16万。(已结题)
6. 中央级公益性科研院所基本科研业务费“牛布鲁氏菌体内感染因子的筛选与鉴定”(项目编号:2010JB16)起止年限:2010.01-2011.12;项目经费:25万。(已结题)
7. 中央级公益性科研院所基本科研业务费“牛布鲁氏菌体内感染因子的功能解析”(项目编号:2012JB05),起止年限:2012.01-2012.12;项目经费:10万。(已结题)

 

代表论著

前3作者的SCI论文(22篇)

[1] Min Yue, Xiangan Han, Leon De Masi, Chunhong Zhu, Xun Ma, Junjie Zhang, Renwei Wu, Robert Schmieder, Radhey S. Kaushik, George P. Fraser, Shaohua Zhao, Patrick F.McDermott, François-Xavier Weill, Jacques G. Mainil, Cesar Arze, W. Florian Fricke,Robert A. Edwards, Dustin Brisson, Nancy R. Zhang, Shelley C. Rankin, Dieter M. Schifferli, Allelic variation contributes to bacterial host specificity. Nature Communications, 2015(Accepted).
[2] Xiangan Han, Lei Liu, Guobo Fan, Yuxi Zhang, Da Xu, Jiakun Zuo, Shaohui Wang, Xiaolan Wang, Mingxing Tian, Chan Ding, Shengqing Yu. Riemerella anatipestifer lacks luxS, but can uptake exogenous autoinducer-2 to regulate biofilm formation. Research in Microbiology, 166(6):486-93, 2015.
[3] Xiangan Han, Hao Bai, Jian Tu, Shaohui Wang, Kezong Qi , Guobo Fan, Yuxi Zhang, Jiakun Zuo, Mingxing Tian, Chan Ding, Shengqing Yu, Deletion of luxS further attenuates the virulence of the avian pathogenic Escherichia coli aroA mutant. Microbial Pathogenesis, 88:39-47, 2015.
[4] Xiangan Han , Xiaoqing Sun, Xueqing Shan, Min Zhang, Jun Song, Mingxing Tian, Guobo Fan, Shaohui Wang, Yongliang Tong, Chan Ding and Shengqing Yu. The in vivo-induced argininosuccinate lyase plays a role on the replication of Brucella abortus in RAW264.7 cells. Microbiology-SGM, 160:567-575, 2014
[5] Xiangan Han, Yongliang Tong, Mingxing Tian, Xiaoqing Sun, Shaohui Wang, Chan Ding, Shengqing Yu. Characterization of the immunogenicity and pathogenicity of malate dehydrogenase in Brucella abortus. World J Microbiol Biotechnol. 30(7):2063-70, 2014
[6] Xiangan Han, Yongliang Tong, Mingxing Tian, Yuxi Zhang, Xiaoqing Sun, Shaohui Wang, Xusheng Qiu, Chan Ding,Shengqing Yu. Enzymatic activity analysis and catalytic essential residues identification of Brucella abortus malate dehydrogenase. The Scientific World Journal ,Volume 2014, 2014
[7] Xiangan Han, Hao Bai, Lei Liu, Hongliang Dong, Jun Song, Qinghai Hu , Chan Ding, Kezong Qi, Haiwen Liu, Shengqing Yu. LuxS gene functions in the pathogenecity of avian pathogenic Escherichia coli. Microbial Pathogenesis, 55,21-27, 2013
[8] Xiangan Han, Chan Ding, Hongjun Chen, Qinghai Hu, Shengqing Yu. Enzymatic and biological characteristics of enolase in Brucella abortus. Molecular Biology Reports, 39 (3), 2705-11, 2012
[9] Xiangan Han , Qinghai Hu, Siyu Ding , Wenjing Chen ,Chan Ding , Liang He , Xiaolan Wang , Jiabo Ding ,Shengqing Yu. Identification and immunological characteristics of chaperonin GroEL in Riemerella anatipestifer. Applied Microbiology and Biotechnology, 93(3),1197-205, 2012
[10] Xiangan Han, Chan Ding, Liang He, Qinghai Hu, Shengqing Yu. Development of loop-mediated isothermal amplification (LAMP) targeting the GroEL gene for rapid detection of Riemerella anatipestifer.Avian Disease, 55(3),379-83, 2011
[11] Xiangan Han , Chengping Lu. Detection of autoinducer-2 and analysis of profile of luxS and pfs transcription in Streptococcus suis serotype 2. Current microbiology, 58 (2),146–152, 2009
[12] Xiangan Han, Chengping Lu. In vitro biosynthesis of autoinducer 2 of Steptococcus suis serotype 2 using recombinant LuxS and Pfs. Enzyme and Microbial Technology , 44 (1), 40–45, 2009
[13] Xiangan Han , Chengping Lu. Biological activity and identification of a peptide inhibitor of LuxS from Streptococcus suis serotype 2. FEMS Microbiology letters, 294(1) ,16–234, 2009
[14] Yue Han, Xiangan Han, Shaohui Wang, Qingmei Meng, Yuxi Zhang, Chan Ding,Shengqing Yu .The waaL gene is involved in lipopolysaccharide synthesis and plays a role on the bacterial pathogenesis of avian pathogenic Escherichia coli. Veterinary Microbiology, 2014.
[15] Wei Jiang, Xiangan Han, Quan Wang, Xintong Li, Li Yi, Yongjie Liu, Chan Ding.Vibrio parahaemolyticus enolase is an adhesion-related factor that binds plasminogen and functions as a protective antigen, Applied Microbiology and Biotechnology, 98(11):4937-48, 2014.
[16] Shoulin Cao, Xiangan Han, Chan Ding, Shaohui Wang, Mingxing Tian, Xiaolan Wang, Wanwan Hou, Jiaping Yue, Guijun Wang, Shengqing Yu. Molecular cloning of the duck mitogen-activated protein kinase 1 (MAPK1) gene and the development of a quantitative real-time PCR assay to detect its expression. Poultry Science, 93(9):2158-67, 2014.
[17] Min Zhang, Xiangan Han, Haiwen Liu, Mingxing Tian, Chan Ding, Jun Song, Xiaoqing Sun, Zongping Liu, Shengqing Yu, Inactivation of the ABC transporter ATPase gene in Brucella abortus strain 2308 attenuated the virulence of the bacteria. Veterinary Microbiology, 164(3-4):322-9, 2013.
[18] Qinghai Hu, Xiangan Han, Xiaojin Zhou, Chan Ding,Yinyu Zhu, Shengqing Yu. OmpA is an important virulence factor of Riemerella anatipestifer, Veterinary  Microbiology, 150 (3-4), 278-283, 2011
[19] Qinghai Hu, Xiangan Han, Xiaojin Zhou, Siyu Ding, Chan Ding, Shengqing Yu. Characterization of biofilm formation by Riemerella anatipestifer. Veterinary Microbiology, 144(3-4): 429-436, 2010
[20] Mingxing Tian, Jing Qu, Xiangan Han, Chan Ding, Shaohui Wang, Daxin Peng,Shengqing Yu. Acid shock protein 24 upregulation in O-antigen export system disrupted Brucella abortus, the mechanism and its function on the bacterial intracellular survival. Infect Immun, 82(7):2840-50, 2014.
[21] Mingxing Tian, Jing Qu, Xiangan Han,Min Zhang, Chan Ding, Jiabo Ding, Guanghua Chen,Shengqing Yu Microarray-Based Identification of Differentially Expressed Genes in Intracellular Brucella abortus within RAW264.7 Cells. PLoS ONE 8(8): e67014. doi:10.1371/journal.pone.0067014, 2013
[22] Qinghai Hu, Jing Tu, Xiangan Han, Yinyu Zhu, Chan Ding,Shengqing Yu. Development of multiplex PCR assay for rapid detection of Riemerella anatipestifer, Escherichia coli, and Salmonella enterica simultaneously from ducks. Journal of Microbiological Methods, 87(1),64-69, 2011

 

第一及通讯作者的中文核心期刊论文(12篇)
[1] 范国博,韩月,张宇曦,韩先干*,王少辉,白灏,孟庆美,祁克宗,丁铲,于圣青.禽致病性大肠杆菌gspL基因缺失株构建及生物学特性分析,微生物学报, 55(1):89-95,2015(* 通讯作者)
[2] 范国博,韩先干*,张宇曦,王少辉,左佳坤,徐达,于圣青*,鸭疫里默氏杆菌pfs基因的克隆表达及单克隆抗体制备,中国动物传染病学报(已接收),2015(* 通讯作者)
[3] 孙晓庆, 韩先干*,宋军,童永亮,单雪芹,丁铲,胡青海,于圣青*, 布鲁氏菌外膜蛋白D15的克隆表达及生物学活性分析,中国预防兽医学报,36(4):273-276,2014(* 通讯作者)
[4] 刘蕾, 韩先干*,刘瑞,白灏,董洪亮,丁铲,刘海文,于圣青*. AI-2对鸭疫里默氏杆菌粘附入侵Vero细胞及其相关基因转录水平的影响,微生物学报, 53(3),313-319,2013(* 通讯作者)
[5] 白灏, 韩先干*,刘蕾,祁克宗,刘海文,丁铲,于圣青*,禽致病性大肠杆菌信号分子AI-2的产生及其luxS和pfs转录水平分析,中国农业科学,46 (15),3220-3226,2013(* 通讯作者)
[6] 韩月,韩先干*,白灏,王少辉,孟庆美,丁铲,于圣青*, rmlA基因缺失影响禽致病性大肠杆菌的生物被膜形成,微生物学报, 53(10),1056-1062,2013(* 通讯作者)
[7] 单雪芹, 韩先干*,张 敏,宋 军,刘海文,田明星,潘 玲,丁 铲,周锦萍,于圣青*,实时荧光定量PCR检测巨噬细胞RAW264.7 中Th1/ Th2型细胞因子转录水平方法的建立及初步应用,细胞与分子免疫学杂志,29(4),430-432,2013(* 通讯作者)
[8] 宋军,单雪芹, 韩先干*,刘佩红,孙晓庆,潘玲,丁铲,于圣青*.布鲁氏菌延胡索酸水合酶与纤连蛋白和纤连蛋白溶酶原的结合活性,中国兽医科学,43(01),2013(* 通讯作者)
[9] 韩先干,白灏,刘蕾,陈文静,丁铲,胡青海,祁克宗,于圣青*.禽致病性大肠杆菌安徽分离株luxS和pfs基因的克隆、表达与细胞外合成AI-2活性检测,微生物学报, 52(9),1167-72,2012
[10] 白灏, 韩先干*, 刘蕾, 单雪芹, 宋军, 刘瑞, 董洪亮, 刘海文, 丁铲, 于圣青*.信号分子AI-2对禽致病性大肠杆菌的调控作用,中国农业科学,45 (24), 5110-5116,2012(* 通讯作者)
[11] 董洪亮,韩先干*,白灏,何亮,刘蕾,刘瑞,柴同杰,丁铲,刘海文,于圣青*.λpL/pR-cI857温控系统的改造及其对大肠杆菌菌蜕制备的影响,生物工程学报,28(12),1423-1430, 2012(* 通讯作者)
[12] 韩先干,何随彬,胡青海,丁铲,于圣青.牛布鲁氏菌GroEL的克隆表达及其生物学特性研究,, 18(5),27-31,2010